FIG. 1.

Assays of CMV- and HIV-specific tetramer staining and IFN-γ ELISPOT responses. Responses detected from an HIV-1-infected infant at 9 (upper panels) and 12 (lower panels) months of age. PBMC were stained with anti-CD8 monoclonal antibody (y axes) and with the relevant HLA class I/peptide tetramer (x axes) or stimulated with peptides in an overnight ELISPOT assay to detect IFN-γ secretion. A2-CMVpp65-NLVPMVATV (left panels) and B8-HIV-1 nef-FLKEDGGL (right panels) responses were detected by both assays. The tetramer-positive CD8⁺ T cells, as percentages of total lymphocytes, are indicated adjacent to each plot. The antigen-specific IFN-γ ELISPOT assay results are shown above each plot.