FIG. 6.
Activation and recruitment of transgenic CTL to the lung were not affected by deficiency in either perforin, FasL, or both effector pathways. Mice were infected with 104 PFU A/Mem, and the total number of transgenic cells in the BAL fluid and spleen was determined by staining with Db/NP366-374 tetramer (•) or for intracellular IFN-γ (○) production following peptide stimulation. Note that transgenic cells from uninfected mice stimulated with cognate peptide under similar conditions did not show any intracellular IFN-γ staining. Data shown are mean ± the standard error of the mean log10 transgenic cells per lung or spleen of three mice.