TABLE 4.
Comparison of titers for glycosylated and nonglycosylated NY99 and infectious-clone-derived WNV variants determined by plaque assay or quantitative Taqman RT-PCR
| Variant | Virus titer at postinfection interval:
|
|||
|---|---|---|---|---|
| 48 ha
|
56 ha
|
|||
| Plaque | Taqman | Plaque | Taqman | |
| NY99 | (7.3 ± 0.4) × 108 | (5.6 ± 0.2) × 108 | (5.5 ± 1.4) × 108 | (6.3 ± 0.3) × 108 |
| NY99ic | (5.5 ± 1.4) × 108 | (5.6 ± 0.2) × 108 | (7.5 ± 1.4) × 108 | (5.4 ± 0.5) × 108 |
| NY99/ETH | (6.0 ± 1.4) × 107 | (2.6 ± 0.1) × 108 | (6.0 ± 0.7) × 107 | (8.4 ± 1.2) × 108 |
| NY99/E154 | (1.3 ± 0.2) × 108 | (4.9 ± 0.2) × 108 | (1.5 ± 0.0) × 108 | (5.0 ± 0.2) × 108 |
a
The samples for 48- and 56-h time points are the same as those presented in Fig. 2a.