FIG. 2.

Construction of the complete HEMV proviral vector pHEMV-TOPO. HEMV was originally cloned and sequenced piecemeal following the successful amplification of the fragments shown from the M. spicilegus genome. The final construct was derived primarily from HEMV6 coupled with the 5′ LTR and 3′ LTR regions. The final construct was ligated into the vector pCR2.1-TOPO. Amplicons acquired and used for initial analysis and sequencing are colored light grey. Amplicons acquired and utilized, or partially utilized, in the final construction of the vector are colored black. The final vector construct was made by ligating together the indicated sequences cut with the indicated restriction endonucleases. Primers used to acquire each amplicon are shown associated with each sequence.