FIG. 1.

Illustration of immunofluorescence staining for keratin, a specific characterization marker for keratinocytes, and CD99. Aliquots of 10⁴ cells were plated in fibronectin-coated six-well plastic tissue culture dishes (BD Biosciences Discovery Labware) and incubated for 72 h. Purity of keratinocytes was then assessed by immunocytochemical analyses with the epithelial cell markers keratin and CD99. Sec Ab, secondary antibody only.