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. 2024 Jan 13;14(9):100937. doi: 10.1016/j.jpha.2024.01.005

Fig. 5.

Fig. 5

Macrophage membrane (Møm)-coated rosuvastatin (RVS)-loaded Prussian blue (PB) nanoparticles (MPR NPs) inhibited pyroptosis in homocysteine (Hcy)-induced macrophages. (A) Flow cytometry and quantitative analysis of reactive oxygen species (ROS)-positive macrophages. (B) Western blot assay and quantification analysis of hypoxia-inducible factor-1 (HIF-1α), nucleotide-binding and oligomerization domain (NOD)-like receptor thermal protein domain associated protein 3 (NLRP3), and apoptosis-associated speck-like protein (ASC) levels. (C) Western blot assay and quantification analysis of pro-caspase-1, caspase-1, and cleaved-gasdermin D (C-GSDMD) protein expression. (D) Immunofluorescence images of NLRP3 and ASC colocalization. (E) Plot profile analysis of red and green fluorescence intensity and Pearson product-moment correlation coefficient analysis (PCCs). (F, G) Immunofluorescence images (F) and fluorescence intensity (G) of caspase-1. (H, I) Immunofluorescence images (H) and fluorescence intensity (I) of C-GSDMD. (J) Western blot assay and quantification analysis of interleukin (IL)-18 and IL-1β protein expression. (K) Enzyme-linked immunosorbent assay (ELISA) assay of inflammatory factor IL-18 and IL-1β. Data are means ± standard deviation (SD) (n = 3). ###P < 0.001 vs. the control group; ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001 vs. Hcy. DAPI: 4′,6-diamidino-2-phenylindole.