Fig. 7.

Macrophage membrane (Møm)-coated rosuvastatin (RVS)-loaded Prussian blue (PB) nanoparticles (MPR NPs) upregulated adenosine triphosphate (ATP)-binding cassette transporter A1 (ABCA1)/ATP binding cassette transporter G1 (ABCG1) and inhabited lipid uptake in macrophages. (A, B) Immunofluorescence images (A) and fluorescence intensity quantification analysis (B) of ABCA1. (C, D) Immunofluorescence images (C) and fluorescence intensity quantification analysis (D) of ABCG1. (E) Cellular uptake of 1,1-dioctadecyl-3,3,3,3-tetramethylindocarbocyanine perchlorate (DiL)-oxidized low-density lipoprotein (ox-LDL). (F) Quantification of DiL-ox-LDL fluorescence intensity. (G, H) Oil red O (ORO) staining (G) and quantitative analysis (H). The above experiments were performed on homocysteine (Hcy)-induced macrophages treated with PB NPs, RVS, and MPR NPs. Data are means ± standard deviation (SD) (n = 3). ^###P < 0.001 vs. the control group; ^∗P < 0.05, ^∗∗P < 0.01, and ^∗∗∗P < 0.001 vs. Hcy. DAPI: 4′,6-diamidino-2-phenylindole.