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Separation of antibody subclasses from polyclonal immune globulin preparations by FPLC. RSVIG and HIVIG were run under identical conditions, and the graphs indicating the protein concentrations of the elution-fractions are superimposed. IgG3 protein was present in the initial flowthrough, as indicated. Upon application of a gradually diminishing pH gradient, IgG2 was eluted, followed and partially overlapped by IgG1. OD, optical density.
