FIG. 2.

HIVIG subclass binding to viral antigens. (A) Binding of unfractionated HIVIG and HIVIG subclasses (or the RSVIG control) to an immunoblot of HIV antigens. The same amount of protein was incubated with each strip. Intact HIVIG and all three subclasses bound to the same bands similarly. (B) Unfractionated HIVIG and HIVIG subclasses demonstrate quantitative differences in binding to HIV lysate by ELISA. Serial dilutions of antibody preparations were applied to HIV lysate-coated ELISA plates and developed in accordance with the manufacturer's instructions. The control immune globulin, RSVIG, did not exhibit binding. Similar results were obtained by using ELISA plates coated with viral envelope (SF2) and V3 peptide (not shown). O.D.₄₅₀, optical density at 450 nm.