FIG. 3.

MDMs secrete pro- and active MMP-9 and MMP-2. MDMs and microglia were cultured as adherent monolayers. Complete medium exchange was performed at the time points indicated, and culture supernatants were tested by zymography after 72 h. A representative zymogram obtained from days 1, 3, 7, and 10 is shown in panel A. Levels of pro-MMP-9 (92 kDa) (B) and pro-MMP-2 (72 kDa) (C) and their active forms (active MMP-9 [D] and active MMP-2 [E], respectively) were quantified densitometrically and compared. Data are representative of a total of six MDM donors and two microglia donors performed three or more times on multiple gels.