FIG. 7.

Cellular localization of MMP-2 in human brain tissue. Fifteen brain autopsy samples were analyzed, including those from 3 control subjects (without CNS disease), 6 patients with severe HIVE, 3 patients with mild HIVE, and 3 HIV-1-seropositive patients without nervous system disease. In the patients with severe HIVE, prominent astrogliosis as identified by GFAP immunoreactivity was observed (A). HIV-1-infected multinucleated giant cells (B) expressed MMP-2 and were prominent in areas with significant monocyte infiltration. Serial sections stained with MMP-2 (C), CD68 (D), HLA-DR (E), and HIV-1 p24 (F) demonstrated activated, HIV-1-infected cells in microglial nodules positive for MMP-2. Control mouse IgG served as the negative control (H). Few MMP-2 antigen-positive endothelial cells were observed in the control samples (G). Sections are stained with GFAP (A), MMP-2 (B, C, and G), HLA-DR (E), HIV-1 p24 (F), and control IgG (H). Immunoreactivity was detected with a Vectastain Elite kit with DAB as a substrate. Tissue sections were counterstained with Mayer's hematoxylin. Original magnification, panels A and C to H, ×200; panel B, ×400.