Fig. 2.

Characterization of m⁶ A modification in Normal and chRCC tissues reveals that decreased methylation number in chRCC Tissues. A, overlap of three biological replicates of m⁶A-SEAL-seq peaks identifying ~ 15,000 high-confident m⁶A peaks corresponding to 11,396 unique transcripts in normal tissues (left); Overlap of three biological replicates of m⁶A-seal peaks identifying ~ 12,800 high-confident m⁶A peaks corresponding to 10,102 unique transcripts in chRCC tissues (right). B, Metagene profile illustrating the region distribution of m⁶A peaks across the indicated mRNA segments. C, Pie chart depicting the fraction of the confident m⁶A peaks in each of the five non-overlapping transcript segments (5’UTR, start codon, coding sequence [CDS], stop codon and 3’UTR) in normal tissues (left); Pie chart depicting RNA types of m⁶A peaks in normal tissues (right). D, Pie chart presenting the fraction of the confident m⁶A peaks in eCRCC tissues (left); Pie chart depicting RNA types of m⁶A peaks in chRCC tissues (right). E, The number of m⁶A peaks in human chromosomes. F, The number of m⁶A peaks per gene. G, The number of m⁶A peaks in five non-overlapping transcript segments