Table 4. Results of our species identification pipeline at three taxonomic levels (Metazoa, order, family) and our morphology and phylogenetic systematics identifications.
| Metazoa-level | Order-level | Family-level | |||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Museum number | Number of mt reads | Number of hits | % reads correct Genus | % reads correct species | Metazoa ID | Percentage of Identity | Number of hits | % reads correct Genus | % reads correct species | Order ID | Percentage of Identity | Number of hits | % reads correct Genus | % reads correct species | Family ID | Percentage of Identity | Morphological ID | Phylogenetic ID CytB | Phylogenetic ID COI |
| EAFIT-M 2126 | 141 | 17 | 64.71 | 47.06 | Myotis riparius | 93.29 | 12 | 91.67 | 66.67 | Myotis riparius | 93.29 | 12 | 100.00 | 66.67 | Myotis riparius | 93.29 | Myotis riparius | Myotis riparius | Myotis riparius |
| EAFIT-M 2106 | 187 | 44 | 65.91 | 0.00 | Nephelomys albigularis* | 96.39 | 41 | 70.73 | 0.00 | Nephelomys albigularis* | 96.39 | 41 | 70.73 | 0.00 | Nephelomys albigularis* | 96.39 | Nephelomys pectoralis | Nephelomys pectoralis | Nephelomys pectoralis |
| EAFIT-M 2143 | 92 | 20 | 65.00 | 5.00 | Histiotus macrotus | 91.80 | 13 | 100.00 | 7.69 | Histiotus macrotus | 91.81 | 13 | 100.00 | 7.69 | Histiotus macrotus | 91.81 | Histiotus humboldti | Histiotus humboldti | Histiotus humboldti |
| EAFIT-M 2107 | 127 | 21 | 47.62 | 47.62 | Handleyomys alfaroi | 96.20 | 19 | 52.63 | 52.63 | Handleyomys alfaroi | 96.20 | 19 | 52.63 | 52.63 | Handleyomys alfaroi | 96.20 | Handleyomys alfaroi | Handleyomys alfaroi | Handleyomys alfaroi |
| EAFIT-M 2124 | 365 | 32 | 53.13 | 15.63 | Histiotus macrotus | 96.36 | 20 | 85.00 | 25.00 | Histiotus macrotus | 96.36 | 21 | 80.95 | 23.81 | Histiotus macrotus | 96.36 | Sturnira bogotensis | Defficient quality sequence | Defficient quality sequence |
| EAFIT-M 2116 | 162 | 18 | 66.67 | 55.56 | Carollia perspicillata** | 96.53 | 13 | 92.31 | 76.92 | Carollia perspicillata** | 96.53 | 13 | 92.31 | 76.92 | Carollia perspicillata** | 96.526 | Carollia brevicauda | Carollia brevicauda | Defficient quality sequence |
| EAFIT-M 2160 | 547 | 45 | 35.56 | 11.11 | Histiotus macrotus | 93.49 | 32 | 56.25 | 15.63 | Histiotus macrotus | 93.49 | 34 | 55.88 | 17.65 | Histiotus macrotus | 93.49 | Anoura geoffroyi | Defficient quality sequence | Defficient quality sequence |
| EAFIT-M 2157 | 194 | 17 | 64.71 | 64.71 | Artibeus lituratus | 97.54 | 11 | 100.00 | 100.00 | Artibeus lituratus | 97.54 | 11 | 100.00 | 100.00 | Artibeus lituratus | 97.54 | Artibeus lituratus | Artibeus lituratus | Artibeus lituratus |
| EAFIT-M 2164 | 99 | 3 | 33.33 | 33.33 | Glossophaga soricina | 99.25 | 1 | 100.00 | 100.00 | Glossophaga soricina | 99.25 | 1 | 100.00 | 100.00 | Glossophaga soricina | 99.25 | Glossophaga soricina | Defficient quality sequence | Defficient quality sequence |
| EAFIT-M 2158 | 441 | 28 | 53.57 | 50.00 | Artibeus lituratus | 95.21 | 17 | 88.24 | 82.35 | Artibeus lituratus | 95.21 | 17 | 88.24 | 82.35 | Artibeus lituratus | 95.21 | Artibeus lituratus | Artibeus lituratus | Artibeus lituratus |
| EAFIT-M 2167 | 195 | 9 | 33.33 | 11.11 | Sturnira bogotensis | 95.66 | 3 | 100.00 | 33.33 | Sturnira bogotensis | 95.66 | 4 | 100.00 | 25.00 | Sturnira bogotensis | 95.66 | Sturnira bogotensis | Sturnira bogotensis | Sturnira bogotensis |
| EAFIT-M 2166 | 323 | 19 | 68.42 | 47.37 | Carollia perspicillata** | 97.60 | 13 | 100.00 | 69.23 | Carollia perspicillata** | 97.60 | 13 | 100.00 | 69.23 | Carollia perspicillata** | 97.60 | Carollia brevicauda | Carollia brevicauda | Defficient quality sequence |
| EAFIT-M 2165 | 817 | 101 | 21.78 | 4.95 | Nephelomys childi | 92.66 | 81 | 28.40 | 7.41 | Nephelomys childi | 92.66 | 86 | 27.91 | 6.98 | Nephelomys childi | 92.66 | Nephelomys childi | Nephelomys childi | Nephelomys childi |
| EAFIT-M 2168 | 287 | 24 | 25.00 | 25.00 | Myotis nigricans** | 93.32 | 8 | 100.00 | 75.00 | Myotis nigricans** | 93.32 | 8 | 100.00 | 75.00 | Myotis nigricans** | 93.32 | Myotis caucensis | Myotis caucensis | Myotis caucencis |
| EAFIT-M 2151 | 569 | 71 | 14.08 | 9.86 | Nephelomys childi | 93.48 | 62 | 32.26 | 22.58 | Nephelomys childi | 93.70 | 59 | 33.90 | 23.73 | Nephelomys childi | 93.70 | Nephelomys childi | Nephelomys childi | Nephelomys childi |
| EAFIT-M 2159 | 390 | 22 | 36.36 | 0.00 | Histiotus macrotus | 94.67 | 22 | 36.36 | 0.00 | Histiotus macrotus | 94.67 | 22 | 40.91 | 0.00 | Histiotus macrotus | 94.67 | Molossus molossus | Defficient quality sequence | Defficient quality sequence |
| EAFIT-M 2150 | 788 | 119 | 57.98 | 9.24 | Akodon sp.** | 97.09 | 103 | 63.11 | 10.68 | Akodon sp.** | 97.09 | 106 | 53.77 | 10.38 | Akodon affinis | 95.841 | Akodon affinis | Akodon affinis | Akodon affinis |
| EAFIT-M 2152 | 157 | 50 | 46.00 | 10.00 | Marmosa robinsoni isthmica | 93.01 | 49 | 28.57 | 14.29 | Marmosa robinsoni isthmica | 93.01 | 49 | 28.57 | 14.29 | Marmosa robinsoni isthmica | 93.01 | Marmosa isthmica | Marmosa isthmica | Marmosa isthmica |
| EAFIT-M 2161 | 464 | 136 | 30.88 | 2.21 | Marmosa robinsoni | 92.38 | 155 | 21.29 | 3.87 | Marmosa robinsoni isthmica | 92.21 | 155 | 21.29 | 3.87 | Marmosa robinsoni isthmica | 92.21 | Marmosa isthmica | Marmosa isthmica | Marmosa isthmica |
| EAFIT-M 2153 | 384 | 35 | 0.00 | 0.00 | Histiotus macrotus | 92.28 | 25 | 0.00 | 0.00 | Histiotus macrotus | 92.28 | 26 | 0.00 | 0.00 | Histiotus macrotus | 92.28 | Zygodontomys brevicauda | Defficient quality sequence | Defficient quality sequence |
Notes:
For each dataset, the number of mitochondrial reads is shown. At every level of analysis, the number of total hits recovered using mtBlaster is included. Species with bolded letters (at the three levels of analysis) correspond to identifications that match the other two lines of evidence (i.e., morphology and phylogenetic analyses). Underlined text correspond to specimens for which the morphological and phylogenetic identifications (When available) did not match the one obtained through our species identification pipeline.
*
Species lacking mitochondrial sequences in GenBank as of March 2023.
**
Species where the most reliable hit corresponds to a misidentified GenBank sequence.