Fig. 3.

Avermectin B1 induces cell apoptosis and cell cycle arrest in osteosarcoma. (A) Flow cytometry was conducted to measure the apoptotic rate of three osteosarcoma cell lines after treatment with IC25 and IC50 of avermectin B1. (B) Quantification of the apoptosis proportion. (C) The cell cycle distribution was detected by flow cytometry after treatment with IC25 and IC50 of avermectin B1. (D) Quantification of the cell cycle percentage. (E) Western blotting was performed to detect the expression levels of the apoptosis-related proteins Bax, BAD, and caspase-9 in avermectin B1-treated cells. (F) The expression levels of the cell cycle-related proteins CDK4, CDK6, Cyclin D1 and Cyclin D2. Data are shown as the means ± SDs with scatter plots from at least three independent experiments. Statistical analysis was performed using one-way ANOVA followed by post-hoc tests. *p < 0.05, **p < 0.01, *** p < 0.001