Fig. 4.

Avermectin B1 induced autophagy in osteosarcoma cells. (A) The LC3 puncta were analyzed by mRFP-GFP-LC3 construct. (B) Quantification of the autophagosomes (yellow dots) and autolysosomes (free red dots). (C) Autophagosome-like structures (indicated by the yellow arrows) were assayed by transmission electron microscopy (magnification, ×6800 and ×13,000). (D) Western blot experiments were performed to measure the expression changes in LC3B and the autophagy-associated proteins ATG7, ATG13 and ATG14. Data are shown as the means ± SDs with scatter plots from at least three independent experiments. Statistical analysis was performed using one-way ANOVA followed by post-hoc tests. **p < 0.01, *** p < 0.001