Fig. 6.

Avermectin B1 inhibits osteosarcoma cell growth and metastasis in vivo. (A) The top panel shows a photograph of a tumor-bearing mouse, and the bottom panel shows a photograph of a subcutaneous tumor. (B) Weight of subcutaneous tumors in the control and avermectin B1 treatment groups in the subcutaneous tumor model. (C) Growth curve of the measured tumor volume in the subcutaneous tumor model. (D, G) Mouse weights separately in the subcutaneous tumor model and lung metastasis model. (E) Lung metastases formed in the avermectin B1-treated and control groups. (F) Lung weight of the lung metastasis model in the control and avermectin B1 treatment groups. (H) Lung metastasis nodules were stained with hematoxylin-eosin and observed by microscopy (magnification, ×100 and ×200). (I) Statistical analysis of lung metastases in the avermectin B1 and control groups in the lung metastasis model. Statistical analysis was performed using Student’s t test. Error bars represent the SD. *p < 0.05, *** p < 0.001