Fig. 7.

Avermectin B1 activates AMPK/ULK1 signaling pathway in human osteosarcoma cells in vivo. (A) Western blot analysis of AMPK, p-AMPK, ULK1, BECN1, LC3B, CDK6 and Caspase 9 in subcutaneous tumor tissues. (B, C) Ki67, CDK6, Caspase 9, p-AMPK and LC3B protein expression in tumor tissues was analyzed by immunohistochemistry (×100 and ×200). (D) Percentage of immunohistochemically positive area for Ki67, CDK6, Caspase 9, p-AMPK and LC3B in subcutaneous tumor tissues. (E) H&E staining images of the heart, liver, spleen, lung and kidney in mice. Data are shown as the means ± SDs with scatter plots from at least six independent experiments. Statistical analysis was performed using Student’s t test. *** p < 0.001