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The variables of cultivation conditions optimized for soluble expression of anti-HER immunotoxin and their symbols and levels (coded and actual values) used in the experimental design
| Symbol | Variable | Levels | Unit | ||
|---|---|---|---|---|---|
| − 1 | 0 | 1 | |||
| A | Post-induction time | 2 | 10 | 18 | h |
| B | Post-induction temperature | 23 | 30 | 37 | °C |
| C | IPTG concentration | 0.1 | 0.55 | 1 | mM |
| D | Culture medium | LB | 2xYT | TB | - |
