Table 3.

Box–Behnken experimental design of 4 factors (A: post-induction time, B: post-induction temperature, C: IPTG concentration and D: culture meium) in 3 levels and corresponding response (soluble anti-HER2 immunotoxin) values

Run	A (h)	B (°C)	C (mM)	D	Response (soluble immunotoxon (µg/ml))	Total immunotoxon (µg/ml)
4	10	30	0.55	LB	199.62	856.566
5	18	30	1	LB	98.123	996.526
6	10	23	0.1	LB	234.928	795.566
7	10	30	0.55	LB	185	897.311
14	2	30	1	LB	85	380.011
18	18	30	0.1	LB	259.535	1127.181
19	10	30	0.55	LB	172.212	842.26
21	18	23	0.55	LB	120	888.344
25	10	37	1	LB	108.271	823.12
30	2	23	0.55	LB	170.671	496.11
33	10	37	0.1	LB	173.21	886.522
34	10	23	1	LB	136.384	645.93
35	2	37	0.55	LB	65.743	573.077
39	2	30	0.1	LB	161.417	609.65
43	18	37	0.55	LB	190.667	1469.989
3	10	30	0.55	TB	166.499	950.44
8	18	37	0.55	TB	178.667	986.127
9	18	30	1	TB	196.321	1339.70
12	2	30	0.1	TB	155.118	477.37
16	2	30	1	TB	80.921	550.639
22	18	23	0.55	TB	80	972.569
27	2	37	0.55	TB	40.554	726.31
29	10	30	0.55	TB	178	877
31	10	37	1	TB	97.654	993.46
36	10	23	1	TB	72.028	758.212
37	2	23	0.55	TB	180.922	849.65
41	10	23	0.1	TB	152.623	786.12
42	10	30	0.55	TB	156	684.883
44	10	37	0.1	TB	76.923	459.666
45	18	30	0.1	TB	63.756	1074.565
1	10	23	0.1	2xYT	200.919	797.16
2	10	37	0.1	2xYT	63.93	282.69
10	18	23	0.55	2xYT	205	870.436
11	10	30	0.55	2xYT	191.914	684.21
13	10	30	0.55	2xYT	173	665
15	18	30	1	2xYT	223.746	1293.674
17	18	30	0.1	2xYT	167.631	984.713
20	2	37	0.55	2xYT	22.418	407.99
23	2	23	0.55	2xYT	114.134	480.111
24	2	30	1	2xYT	70.324	411.862
26	10	37	1	2xYT	115.249	911.36
28	10	30	0.55	2xYT	180	634.28
32	18	37	0.55	2xYT	174.668	1258.144
37	2	23	0.55	TB	180.922	849.65
40	2	30	0.1	2xYT	152	740.11