Table 4.
Analysis of variance (ANOVA) and regression coefficients of the developed response surface quadratic model for soluble expression of anti-HER2 immunotoxin regarding the influence of post-induction time (A), post-induction temperature (B), IPTG concentration (C) and culture medium (D)
| Source | Sum of squares | Df | Mean square | F-value | p-value | |
|---|---|---|---|---|---|---|
| Model | 1.36E + 05 | 29 | 4674.13 | 14.74 | < 0.0001 | Significant |
| A-Time | 18089.11 | 1 | 18089.11 | 57.03 | < 0.0001 | Significant |
| B-Temp | 8172.32 | 1 | 8172.32 | 25.77 | 0.0001 | Significant |
| C-Conc | 10199.15 | 1 | 10199.15 | 32.16 | < 0.0001 | Significant |
| D-Culture | 7851.57 | 2 | 3925.79 | 12.38 | 0.0007 | Significant |
| AB | 18882.44 | 1 | 18882.44 | 59.54 | < 0.0001 | Significant |
| AC | 5614.2 | 1 | 5614.2 | 17.7 | 0.0008 | Significant |
| AD | 7915.93 | 2 | 3957.96 | 12.48 | 0.0006 | Significant |
| BC | 7698.9 | 1 | 7698.9 | 24.27 | 0.0002 | Significant |
| BD | 1246.78 | 2 | 623.39 | 1.97 | 0.1745 | Not significant |
| CD | 10989.26 | 2 | 5494.63 | 17.32 | 0.0001 | Significant |
| A² | 2990.26 | 1 | 2990.26 | 9.43 | 0.0078 | Significant |
| B² | 12045.68 | 1 | 12045.68 | 37.98 | < 0.0001 | Significant |
| C² | 3903.63 | 1 | 3903.63 | 12.31 | 0.0032 | Significant |
| ABD | 4052.39 | 2 | 2026.19 | 6.39 | 0.0098 | Significant |
| ACD | 11626.06 | 2 | 5813.03 | 18.33 | < 0.0001 | Significant |
| BCD | 2292.19 | 2 | 1146.09 | 3.61 | 0.0524 | Not significant |
| A²D | 1124.51 | 2 | 562.25 | 1.77 | 0.2036 | Not significant |
| B²D | 1357.49 | 2 | 678.75 | 2.14 | 0.1522 | Not significant |
| C²D | 1410.5 | 2 | 705.25 | 2.22 | 0.1426 | Not significant |
| Residual | 4757.38 | 15 | 317.16 | |||
| Lack of Fit | 3956.16 | 9 | 439.57 | 3.29 | 0.0803 | Not significant |
| Pure Error | 801.22 | 6 | 133.54 | |||
| Cor Total | 1.40E + 05 | 44 |