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. 2024 Sep 28;15:8425. doi: 10.1038/s41467-024-52739-y

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 3 — a Schematic diagram for the construction of myofibroblast ASPP1 KO mice. ASPP1^fl/fl mice were crossed with Postn^MCM to obtain ASPP1^fl/fl Postn^MCM mice. b Schematic diagram of the experimental design. ASPP1^+/+Postn^MCM (Postn^MCM) and ASPP1^fl/fl Postn^MCM (ASPP1-CKO) mice were given TAM continuously for 5 days after MI by intraperitoneal injection. Postn^MCM, ASPP1^+/+ Postn^MCM; ASPP1-CKO, ASPP1^fl/fl Postn^MCM; TAM, tamoxifen. The diagram was created using Figdraw. c ASPP1 protein level in the heart of ASPP1^fl/fl Postn^MCM by western blot assay, n = 6 independent samples. d Kaplan–Meier analysis of the survival of ASPP1^+/+Postn^MCM (Postn^MCM) and ASPP1-CKO upon MI 4 weeks. n = 24 in Postn^MCM + MI group, n = 37 in ASPP1-CKO + MI group. e Echocardiographic measurement of cardiac function. n = 18 in Postn^MCM group, n = 16 in ASPP1-CKO group. f Representative images of the hearts and quantification of the ratio of HW to BW in four groups of mice. n = 8 in Postn^MCM group, n = 7 in ASPP1-CKO group, n = 8 in Postn^MCM + MI group, n = 7 in ASPP1-CKO + MI group, Scale bar = 1 mm. Magnification 2×. g Coronal cross-section images of Masson’s trichrome staining and statistical analysis of fibrotic area (infarct size) using Image-Pro Plus. n = 7 in the Postn^MCM group, n = 7 in the ASPP1-CKO group, n = 7 in the Postn^MCM + MI group, n = 10 in ASPP1-CKO + MI group. Scale bar = 1 mm. Magnification 2× in the whole heart view group. Scale bar = 5 mm, Magnification 20× in border area part. h, i. Transcriptional level of genes encoding ECM (h) and myofibroblast markers molecules (i) in left ventricular tissue at 28 days after MI by qRT-PCR, n = 6 independent samples. Data are represented as mean ± SEM. Statistics: A two-tailed Student’s t-test was used to calculate the presented P values (c). Log-rank (Mantel–Cox) test (d). 2-way ANOVA followed by Tukey post hoc test (e). One-way ANOVA, followed by Tukey post hoc multiple comparisons test (f–i). Source data are provided as a Source Data file.