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. 2024 Sep 28;15:8425. doi: 10.1038/s41467-024-52739-y

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 5 — a p53 protein levels in ASPP1-KO group (The left) and ASPP1-CKO group (the right) by western blot assay, n = 6 independent samples in ASPP1-KO group in each group, n = 6 independent samples in ASPP1-CKO group in each group. b Effects of ASPP1 knockdown (si-ASPP1) on TGF-β1-induced (The left) or ASPP1 overexpression (The right) negative regulation of p53 protein levels in PMCFs by western blot analysis, n = 6 independent samples. c Effects of ASPP1 knockdown (si-ASPP1) on TGF-β1-induced (The left) or ASPP1 overexpression (the right) on p53 mRNA levels in PMCFs by qRT-PCR, n = 8 independent samples in si-ASPP1 + TGF-β1 group, n = 7 independent samples in ASPP1 overexpression group. d, e qRT-PCR was used to evaluate the expression of genes encoding cell cycle regulators in PMCFs, n = 6 independent samples in each group. f, g Immunofluorescent staining of ASPP1 and p53 in PMCFs. ASPP1 was stained in red, and p53 in green. The nucleus was stained in blue with DAPI, n = 3 independent experiments. Scale bar = 20 μm. Magnification 60×. h The protein levels of Col1 and Fn1 after co-transfection of si-ASPP1 and the siRNA for p53 (si-p53), n = 5 independent samples in each group. i The effect of Col1 and Fn1 after co-transfection of oe-ASPP1 plasmids and p53 overexpression (oe-p53) plasmids, n = 5 independent samples in each group. Data are represented as mean ± SEM. Statistics: A two-tailed Student’s t-test was used to calculate P values (a–e). One-way ANOVA, followed by Tukey post hoc multiple comparisons test (h, i). Source data are provided as a Source Data file.