FIG. 1.

Analysis of binding of biotinylated IgG1 b12 to B2.1 phage (B2.1φ) and B2.1 synthetic peptide (B2.1pep) by ELISA. Competition for IgG1 b12 binding to plate-adsorbed B2.1 phage by the following in-solution competitors is shown: 2 × 10¹⁰ B2.1 phage, 300 μM B2.1 synthetic peptide, 100 nM gp120_Ba-L (gp120), f88-4 phage (f88 φ), and unrelated peptide G45B, whose sequence is VERSKAFSNCYPYDVPDYASLRS. BSA is bovine serum albumin, and n. c. indicates no in-solution competitor. O.D._405–490, optical density at 405 minus 490 nm.