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Titration of Fab b12 (A), IgG1 b12 (B), and murine anti-B2.1 peptide serum (C) on different immobilized antigens. Twofold dilutions of Fab and IgG1 b12 and fourfold mouse serum dilutions were reacted with biotinylated B2.1 directly adsorbed to ELISA wells (bio-B2.1), biotinylated B2.1 bound to immobilized streptavidin (SA+bio-B2.1), gp120Ba-L, B2.1 recombinant phage, f88-4 phage, bovine serum albumin (BSA), ovalbumin, and streptavidin (SA). O.D., optical density.
