TABLE 1.

Sequences and ELISA signals of peptide phage clones affinity selected by biotinylated IgG1 b12

Clone	Peptide sequencea	OD405–490e
		IgG1 b12	Fab b12
		37°C	4°C	37°C
Ed1	T C LW SDL RAQ CI	NDb	ND	ND
B1 library	X C XX SDL XXX CI
B1.2	G C LY SDL LAT CI	1.321	0.013	0.019
B1.11	N C LY SDL TQS CI	1.236	0.016	0.018
B1.9	N C LY SDL YAR CI	1.223	0.013	0.015
B1.20	K C MY SDL LGI CI	1.153	0.012	0.021
B1.10	D C LY SDL ESR CI	0.818	0.015	0.021
B1.4	S C LY SDL LEL CI	0.750	ND	ND
B1.3	E C MW SDL ELR CI	0.571	ND	ND
B1.12	N C LW SDL EQF CI	0.343	ND	ND
Ed2	REKRWIF SDL THT CI	ND	ND	ND
B2 library	XXXXXXX SDL XXX CI
B2.1	HERSYMF SDL ENR CI	1.236	1.071	0.219
B2.11	CSRNQLW SDL HGS CI	1.207	0.012	0.016
B2.12	NNQGCLW SDL TAS CI	1.189	0.013	0.017
B2.18	STTRCTW SDL YDS CI	1.141	0.011	0.016
B2.8	QSSSCMW SDL FQQ CI	0.992	0.016	0.018
B2.6	AQKQCTW SDL LSR CI	0.903	0.019	0.018
B2.7	RPCRGVY SDL LDK CI	0.886	0.016	0.020
B2.10	SSDHCLW SDL TMT CI	0.644	ND	ND
B2.3	LPSSCSW SDL LNR CI	0.276	ND	ND
B2.15	HTCAGTW SDL LST CI	0.252	ND	ND
gp120c		1.121	0.863	1.166
f88-4d		0.075	0.014	0.019

^aBold residues indicate the fixed residues in the sublibraries. 

^bND experiment not done. 

^cPositive control. 

^dNegative control. 

^eOD_405–490, optical density at 405 minus 490 nm.