TABLE 2.
Percent yields of four successive rounds of affinity selection of phage-displayed Fab library M with B2.1 phagea
| Round and phage or protein immobilized on plate | Input (TU, 109) | Output (TU, 104) | % Yield |
|---|---|---|---|
| 1 | |||
| B2.1 | 62 | 4.8 | 9.2 × 10−5 |
| f88-4 | 62 | 4.0 | 7.8 × 10−5 |
| gp120 | 62 | 9.6 | 1.8 × 10−4 |
| 2 | |||
| B2.1 | 6.6 | 2.4 | 3.6 × 10−4 |
| f88-4 | 6.6 | 1.6 | 2.4 × 10−4 |
| gp120 | 6.6 | 140 | 2.1 × 10−2 |
| 3 | |||
| B2.1 | 2.1 | 14 | 6.8 × 10−3 |
| f88-4 | 2.1 | 14 | 6.8 × 10−3 |
| gp120 | 2.1 | 3,200 | 1.5 |
| 4 | |||
| B2.1 | 2.7 | 400 | 1.5 × 10−1 |
| f88-4 | 2.7 | 19 | 8.8 × 10−3 |
| gp120 | 2.7 | 110 | 4.1 × 10−1 |
a
For panning, 400 ng of gp120SF2 and 5 × 1010 recombinant B2.1 or f88-4 phage were immobilized on a plate. Input and output phage values are given in ampicillin-resistant transfecting units (TU).