Comparison of calcium signal between axonal branches
(A) Example of the normalized calcium peak fluorescence as a function of stimulus frequency at a branch point; parent branch (yellow), and two secondary branches (cyan and magenta).
(B) Normalized integrated calcium fluorescence as a function of stimulus frequency.
(C–N) Pooled differences between normalized signals (black vertical lines in A), for peak (upper row) and area under the curve (bottom row) across different spike train frequencies (n = 17). Red bars indicate the difference values from the example shown in A and B.