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. 2024 Sep 16;15:1458967. doi: 10.3389/fimmu.2024.1458967

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Copyright © 2024 Govaerts, Van Breedam, De Beuckeleer, Goethals, D’Incal, Di Stefano, Van Calster, Buyle-Huybrecht, Boeren, De Reu, Paludan, Thiry, Lebrun, Sadzot-Delvaux, Motaln, Rogelj, Van Weyenbergh, De Vos, Vanden Berghe, Ogunjimi, Delputte and Ponsaerts

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Longitudinal characterisation of NSPH differentiation. (A) Representative images of NSPHs at the age of 1, 2, 3 and/or 5 months immunolabelled for the neuronal markers Tuj1 (magenta), MAP2 (magenta) and NeuN (magenta), and the late-stage apoptosis TUNEL staining (magenta), as indicated. (B) Representative images of NSPHs at the age of 1, 2, 3 and/or 5 months immunolabelled for the astrocyte markers GFAP (magenta), S100b (magenta), AQP4 (magenta) and SOX9 (magenta), as indicated. Nuclei are labelled with DAPI (blue). Scale bars of 20, 50 and 500 µm are indicated on the images.