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. 2024 Sep 16;15:1458967. doi: 10.3389/fimmu.2024.1458967

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Copyright © 2024 Govaerts, Van Breedam, De Beuckeleer, Goethals, D’Incal, Di Stefano, Van Calster, Buyle-Huybrecht, Boeren, De Reu, Paludan, Thiry, Lebrun, Sadzot-Delvaux, Motaln, Rogelj, Van Weyenbergh, De Vos, Vanden Berghe, Ogunjimi, Delputte and Ponsaerts

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Formation of stress granules in VZV^eGFP-ORF23 and SeV^eGFP-infected NSPHs. Representative overview immunofluorescence images of control NSPHs [(A), top left], NSPHs inoculated with eGFP+ control ARPE19 cells [(A), top right, ARPE-CTRL], NSPHs inoculated with VZV^eGFP-ORF23-infected ARPE19 cells [(B), top left, VZV^eGFP-ORF23], and NSPHs infected with SeV^eGFP [(C), top right, SeV^eGFP], immunolabelled for G3BP1 (orange) and PABPC1 (magenta). Insets of virus-infected cells are depicted by white arrows on the overview image [(B, C), top] and shown below the corresponding overview images [(B, C), bottom]. Nuclei are labelled with DAPI (blue). Scale bars of 50 (overview) and 5 (detail) µm are indicated on the images.