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. 2024 Sep 16;15:1458967. doi: 10.3389/fimmu.2024.1458967

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Copyright © 2024 Govaerts, Van Breedam, De Beuckeleer, Goethals, D’Incal, Di Stefano, Van Calster, Buyle-Huybrecht, Boeren, De Reu, Paludan, Thiry, Lebrun, Sadzot-Delvaux, Motaln, Rogelj, Van Weyenbergh, De Vos, Vanden Berghe, Ogunjimi, Delputte and Ponsaerts

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VZV^ORF65-tdT-66 affects cellular integrity in infected NSPHs. (A) Representative images of cryosections of control (CTRL) and VZV^ORF65-tdT-66 infected NSPH at day 7 post-infection (dpi), in which nuclei are labelled with DAPI (blue) and infection with VZV^ORF65-tdT-66 is shown in red (direct tdTomato fluorescence signal). (B) Representative images Haematoxylin-Eosin stained cryosections of control (CTRL) and VZV^ORF65-tdT-66 infected NSPH at 7 dpi. (C) Representative immunofluorescence images of control (CTRL) and VZV^ORF65-tdT-66 infected NSPH at 7 dpi, immunolabelled for the neuronal marker MAP2 (green, top panel) and astrocyte marker GFAP (green, bottom panel). Nuclei are labelled with DAPI (blue). Scale bars of 100 (overview) and 50 (detail) µm are indicated on the images.