FIGURE 3.

Oleuropein decreases the aggregated and soluble forms of truncated mHtt in N2a cells. (A) Immunoblot detection of the aggregated and soluble forms of mutant Htt (HttQP72-GFP) in N2a cells transfected with the HttQP72-GFP plasmid in the absence or presence of oleuropein treatment at the indicated concentration for 24 or 48 h. Cells were treated with 100 μg/ml cycloheximide (CHX) to inhibit protein synthesis. Actin was used as an internal control. (B) Quantification analyses of the aggregated form of HttQP72-GFP protein. (C) Quantification analyses of the soluble form of HttQP72-GFP protein. Actin was used for normalization. Data from three independent experiments are presented as mean normalized units ± SEM. Data showing significant differences are labeled as follows: p < 0.05 with one asterisk (*) and p < 0.01 with two asterisks (**).