FIG. 6.

Immunofluorescence microscopy of vE120Ri-infected cells. Vero cells infected with recombinant vE120Ri virus in the presence (A and B) or absence (C and D) of IPTG were fixed at 18 hpi and double-labeled with rabbit serum anti-pE120R (A and C) and mouse MAb 17L.D3 anti-p72 (B and D). Labeling was revealed with Alexa 488 goat anti-rabbit rabbit IgG and with Alexa 594 goat anti-mouse IgG. Insets in panels A and B show enlarged images of the delimited cytoplasmic areas. Viral factories and virions spread throughout the cytoplasm are indicated by arrows and arrowheads, respectively.