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. 2024 Sep 12;14(9):e085504. doi: 10.1136/bmjopen-2024-085504

Table 1. Summary of HOMISPEC laboratory Procedures.

Study subject Research sample type Time points Sample quantity Sample transport to biorepository Sample collection Processed sample Sample aliquots Storage temperature
Disease cohort (liver disease and haematological malignancy) Peripheral blood Baseline, week 1, 2, 3, 4, month 3, 6, 9, 12, 24 18 mL 2–8°C, ice packs 9 mL EDTA Plasma 6 × 1.5 mL aliquots −80°C
PBMC 5 × 5 × 106/1 mL aliquots Liquid nitrogen
Stool Baseline, week 1, 2, 3, 4, month 3, 6, 9, 12, 24 25 g Ambient Sterile sample contains with preservation material DNA/RNA ShieldTM 6 × 1 mL aliquots −80°C
OMNI met®•GUT | ME-200 2 × 0.5 mL aliquots
Saliva Baseline, week 1, 2, 3, 4, month 3, 6, 9, 12, 24 2 mL Ambient Sterile sample contains with preservation material DNA/RNA ShieldTM 2 × 1 mL aliquots −80°C
Bronchoalveolar lavage Time of event requiring clinically directed BAL 40–80 mL 2–8°C, ice packs Sterile sample container without preservation material DNA/RNA ShieldTM 20 × 1.5 mL aliquots −80°C
Colonoscopy Time of event requiring clinically directed colonoscopy 40–80 mL 2–8°C, ice packs Sterile sample container without preservation material Stool—DNA/RNA ShieldTM 10–20 × 1.5 mL aliquots −80°C
1–4 tissue samples 2–8°C, ice packs Sterile sample container preservation material Tissue—DNA/RNA ShieldTM 1–4 × biopsy samples
Healthy controls Peripheral blood Baseline 18 mL 2–8°C, ice packs 9 mL EDTA Plasma 6 × 1.5 mL aliquots −80°C
PBMC 5 × 5 × 106/1 mL aliquots Liquid nitrogen
Stool Baseline 25 g Ambient Sterile sample contains preservation material DNA/RNA ShieldTM 6 × 1 mL aliquots −80°C
OMNI met®•GUT | ME-200 2 × 0.5 mL aliquots
Saliva Baseline 2 mL Ambient Sterile sample contains preservation material DNA/RNA ShieldTM 2 × 1 mL aliquots −80°C
Deceased organ donors Ascending and descending colon Time of organ donation 10–20 cm of en bloc resected colon sample 2–8°C, ice packs Sterile sample contains with preservation material Stool—DNA/RNA ShieldTM 6–12 × 1.5 mL aliquots −80°C
Stool - OMNI met®•GUT | ME-200 4 × 0.5 mL aliquots
Stool—Glycerol 4–6 2 mL aliquots
Tissue—DNA/RNA ShieldTM 4 × 1–3 cm tissue aliquots
Bile Time of organ donation 5–20 mL 2–8°C, ice packs Sterile sample container without preservation material DNA/RNA ShieldTM 6 × 1.5 mL aliquots −80°C
OMNI met®•GUT | ME-200 2–4 × 0.5 mL aliquot
Glycerol 2–4 × 0.5 mL aliquot