Overexpression of circMyst4 in vivo alleviated the symptoms of hypoxia-mediated pulmonary hypertension
(A and B) Right ventricular systolic pressure (RVSP), and right ventricle (RV)/left ventricle (LV)+S weight ratio in the Nor+NC, Nor+AAV5, Hyp+NC, and Hyp+AAV5 groups are shown (n = 6 mice/group).
(C–E) Echocardiographic analysis of pulmonary artery acceleration time (PAAT), pulmonary artery velocity time integral (PAVTI) and cardiac output (CO) in the different groups (n = 6 mice/group).
(F) Morphological analysis of the pulmonary artery was performed using HE staining (n = 6 mice/group). Scale bar, 50 μm. Data are shown as means ± SD. Statistical analysis was performed with one-way ANOVA followed by Bonferroni correction. Hyp, hypoxia; Nor, normoxia; NC, negative control; AAV5, serotype 5 adenovirus-associated virus carrying circMyst4; ns, not significantly different. ∗∗p < 0.01, ∗∗∗p < 0.001.