Skip to main content
. 2024 Sep 12;27(10):110900. doi: 10.1016/j.isci.2024.110900

Figure 3.

Figure 3

CircMyst4 overexpression attenuates PASMC ferroptosis induced by hypoxia

(A) Western blotting analysis of the protein levels of GPX4, ACSL4 and ptgs2 in PASMCs (n = 6).

(B) Immunofluorescence analysis of GPX4 expression. Scale bars, 100 μm (n = 6). Green color denotes GPX4, stained with FITC, and blue color denotes nucleus, stained with DAPI.

(C–E) Detection of the GSH depletion, MDA release and ferrous ion accumulation in PASMCs (n = 6).

(F) Detection of the percentage of ferrous ion staining-positive cells by a specific probe of ferrous ion (red). Scale bars, 100 μm (n = 6).

(G) Representative electron micrograph of PASMCs in different groups. Asterisks represent mitochondrion. Scale bars, 0.5μm (n = 3). Data are shown as means ± SD. Statistical analysis of the graph (A) and (D) was performed with one-way ANOVA followed by Dunn’s post-test. Statistical analysis of the graph (C) and (E) was performed with one-way ANOVA followed by Bonferroni correction. Hyp, hypoxia; Nor, normoxia; NC, negative control. ∗∗p < 0.01.