Skip to main content
. 2024 Sep 12;27(10):110900. doi: 10.1016/j.isci.2024.110900

Figure 8.

Figure 8

CircMyst4 decreases pulmonary fibrosis levels by regulating ferroptosis

(A and B) Masson staining and immunofluorescence analysis of mouse pulmonary fibrosis of in different groups. Scale bar, 100μm (n = 6). Red color denotes collagen-III or α-SMA, stained with Cy3, green denotes fibronectin or α-SMA, stained with FITC and blue color denotes nucleus, stained with DAPI.

(C and D) After overexpression with circMyst4, western blotting and immunofluorescence analysis of the levels of collagen III and fibronectin. Scale bar, 100μm (n = 6). Red color denotes collagen-III, stained with Cy3, and blue color denotes nucleus, stained with DAPI.

(E and F) After Ferrostatin-1 (an inhibitor of ferroptosis, Fer-1) treatment, western blotting and immunofluorescence analysis of the levels of collagen-III and fibronectin. Scale bar, 100μm (n = 6). Red color denotes collagen-III, stained with Cy3, and blue color denotes nucleus, stained with DAPI.

(G and H) After circMyst4 overexpression and Rsl3 (an inducer of ferroptosis) treatment, western blotting and immunofluorescence analysis of the levels of collagen-III and fibronectin. Scale bar, 100μm (n = 6). Red color denotes collagen-III, stained with Cy3, and blue color denotes nucleus, stained with DAPI. Data are shown as means ± SD. Statistical analysis of the graph (C, E, and H) was performed with one-way ANOVA followed by Dunn’s post-test. Hyp, hypoxia; Nor, normoxia; NC, negative control; AAV5, serotype 5 adenovirus-associated virus carrying circMyst4. ∗p < 0.05, ∗∗p < 0.01.