FIG. 2.

Expression of Gag-PR in E. coli and demonstration of autoprocessing. (A) Schematic representation of the expression constructs used to generate the underlying blots. The carboxy-terminal hexahistidine tag is shown in black. Relevant amino acid positions are shown. (B) Immunoblot of bacterial lysates in which the Gag-PR constructs were expressed. VLPs from yeast were separated in lane 1. (C) Appearance of the PR following expression of the PPR precursor protein. Polyclonal antibody R2-F, used in the immunoblot shown in panel B, recognizes Ty1 Gag. Antipeptide antibody JH695 (15), directed against an epitope in the p4 region, was used in the immunoblot shown in panel C.