TABLE 1.
Oligonucleotides used in this study
| Oligonucleo- tide | Sequencea |
|---|---|
| JB30 | CGACCTTCACTTGGGCCAGGAAC |
| JB1329 | CTTGTATGCATATGCTAATTGATGGTGGGTACGGAGA |
| JB1378 | AGCCTTTACATATGGAATCCCAACAATTATCTCAAC |
| JB1553 | CTCCTTCTCGAGGCAGCAGCATCACGA |
| JB1890 | GCGGATCCGAAGAACAACAGGGATCGAGAAAC |
| JB1891 | GCGGATCCTTAATGATGATGATGATGATGATTGATGGTGGGTACGGAGATATT |
| JB1992 | GCGGATCCGCGTCTGATGATGAACTCCCTGGA |
| JB1993 | ATAATGTCAGACCACCACCAAT |
| JB1994 | TGTCCTGGAAGTGAAATTGTA |
| JB2016 | GCGGATCCCATCTTAGGCCATAGCACGGACAACGATTCCATC |
| JB2017 | GCGGATCCGGATACATTGTGAGCCCTGGC |
| JB2018 | TTCCGGATCCCATCTTAGGCCATGAACCGATTCAATTGAACAATAAG |
| JB2019 | TATGGGATCCGGAATCGTTGTCCGTG |
| JB2531 | GCGGATCCGCTGCTCTTAGGCCAGAAACTTAC |
| JB2537 | AGCAGCCGATCCAGCAGCAGTAGTTGATTTACTGATGG |
| JB2538 | GCTGCTGGATCGGCTGCTCTGGGCCAGAAACTTACTG |
| JB2539 | AGCAGCCGATCCAGCAGCGTTCAATTGAATCGGTTC |
| JB2626 | CCATCAGTAAATCAACTACTGCAGCCGGCTCCGCAGCAAATAAGCACGACCTTCAT |
| JB2628 | TGAACCGATTCAATTGAACGCAGCCGGCTCCGCAGCACTGGGCCAGAAACTTACT |
a
Restriction sites are underlined. Nucleotides that introduce mutations are in boldface.