Figure 4.

Metastatic state of MDA-MB-231 cells affects cellular real-time responses to DTX treatment. (a) ΔF-responses and change in frequency of MDA-MB-231 cells with varying expression of Cx43 to DTX (20 and 50 nM) (b) ΔD-responses and change in dissipation of MDA-MB-231 cells with varying expression of Cx43 to DTX (20 and 50 nM). The values depicted are the mean ± SEM from at least three separate experiments evaluated per condition. (c) Confocal images of treated cells at different time points. The cells were stained with actin phalloidin (red), and the nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI) (blue). Scale bar: 20 μm. (d) Cell area (μm²) progression upon treatment with DTX at different time points.