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(a–d) Far-UV CD spectra of RgPAL in buffer and ILs as a function of temperature, (e) corresponding sigmoidal curve of temperature-dependent fraction of unfolded enzyme depicting two-state transition. (f) Represents the stability curve of RgPAL followed by (g) vant-Hoff plot leading to (h) the values of change in enthalpy (ΔH) and entropy (ΔS) of protein unfolding in buffer and IL+Tris-HCl systems.
