FIG. 2.

C protein expression inhibits SeV mRNA synthesis in transfected cell extracts. Viral mRNA synthesis was carried out with purified N:RNA nucleocapsids and cytoplasmic extracts of vTF7-3 infected A549 cells that had been transfected with pGEM-L and various pGEM-P/C plasmids (as indicated above), in the presence of actinomycin D and [α-³²P]GTP (see Materials and Methods). P/C^stop contains a stop codon just downstream of AUG²⁰¹ (Y2) and does not express any of the C proteins; none, control (no pGEM-P/C). The products of the reaction were purified by Trizol extraction and separated on a 1.5% formaldehyde-agarose gel. The N and P mRNA bands were quantified in a PhosphorImager, and their relative intensities are shown below.