Skip to main content
. 2024 Sep 30;17:238. doi: 10.1186/s12920-024-02012-4

Table 1.

Primer sequences used to analyze the variant of the COL4A2 gene and vector pcDNA3.1

Primer name Primer sequence (5` − 3`)
120,011-COL4A2-F CGTTGCGGGTGTGTCTTCTC
120,334-COL4A2-F CCCAGGTGAAGCAGAACTTT
122,754-COL4A2-R GGATGACCGGACGATGGTCC
123,097-COL4A2-R ATATGCCTGCTAGCATGGGT
pcDNA3.1-COL4A2-KpnI-F GCTTGGTACCATGGGACACCCGGGGCAAGGTGG
COL4A2-mut-F ACAGATATCGGGTACATTTGCAAGAGATGGG
COL4A2-mut-R CCCATCTCTTGCAAATGTACCCGATATCTGT
pcDNA3.1-COL4A2-EcoRI-R TGCAGAATTCCTGGAAACCGACCAATCCAG
pcDNA3.1-F CTAGAGAACCCACTGCTTAC
pcDNA3.1-R(BGH-R) TAGAAGGCACAGTCGAGG