TABLE 1.

Relative amounts of ³⁵S-sBPP bound to B- and C-capsids isolated from the nuclear and cytoplasmic fractions of SCMV-infected cells

Virus particle	35S-sBPPa (A)	Triplexb (B)	35S-sBPP/triplex (A/B)	Ratioc
Nuclear
B-capsid	12,402	24,540	0.50	≥10:1
C-capsid	≤150	3,130	≤0.05
Cytoplasmic
B-capsid	1,553	2,190	0.71	4.7:1
C-capsid	355	2,344	0.15

^aPhosphorimaging measurements were made from the CBB-stained dried gels shown in Fig. 3. The sBPP bands quantified are from lanes 9, 10 (nuclear B-capsids), and 12 (nuclear C-capsids) of Fig. 3A and lanes 8, 9 (cytoplasmic B-capsids), and 11 (cytoplasmic C-capsids) of Fig. 3B. The numbers are in units of photostimulated luminescence (PSL; proportional to the amount of radioactivity present) and are corrected for background. 

^bPhosphorimaging measurements were made from the bands shown in the Fig. 3 insets. Triplex protein bands (i.e., mCP and mCBP, in the lanes specified in footnote a) were quantified separately and corrected for background, and the PSL values were combined to give the numbers shown. The triplex proteins are in a fixed copy number in capsids (15) and were used to normalize the ³⁵S-sBPP measurements. Triplex values for nuclear and cytoplasmic capsids were obtained from the same coprocessed membrane and are directly comparable. 

^cCalculated as (A/B for B-capsids) divided by (A/B for C-capsids).