Fig. 3. The hrPKS and α,β-hydrolase collaboratively produce α-pyrone-polyketide precursor 7. (a) Two proposed pathways for the collaboration of hrPKS ProF and α,β-hydrolase ProG. (b) LC-MS analysis of the culture extracts from AN-proF and AN-proFG. (c) The yields of 7 increased nearly fourfold in AN-proFG indicating that ProG plays a supportive role with ProF in the biosynthesis of 7.
