FIG. 5.

AAV5 transduction of resialylated sialic acid-deficient Lec2 and parental Pro5 cells. Lec 2 cells are a mutant clone derived from the parental Pro5 cell line that is deficient in the transport of CMP-sialic acid into the Golgi compartment. The following sialyltransferases were used to add specific sialic acids to the surface of the Lec2 cells: ST-2,3 (O) sialyltransferase, ST-2,3 (N) sialyltransferase, or ST-2,6 (N) sialyltransferase. The resialylation was confirmed by specific lectin adsorption (data not shown). Resialylated Lec2 cells were then transduced (MOI, 1) with either recombinant AAV5 or AAV4 and β-Gal activity was measured 24 h posttransduction using a chemiluminescent assay. (A) Transduction with AAV5 (n = 4); (B) transduction with AAV4 (n = 3).