FIG. 3.

Recombination between linear molecules sharing different lengths of overlapping end sequence identity in vaccinia virus-infected cells. Each of 12 different PCR-amplified inserts (LucM0 to LucM333) plus a cut vector (pRP406/BstEII+PacI) were cotransfected into vaccinia virus-infected cells and the recombinant frequencies were calculated in two separate experiments as described in Materials and Methods. The cross is shown inset, with gray boxes indicating the luciferase open reading frame. Control experiments detected 0.055 and 0.035% luciferase activity in cells transfected with just LucM333 or pRP406/BstEII+PacI DNAs, respectively, relative to that of cells transfected with the intact gene. Results from separate replicate experiments are shown.