TABLE 1.
Primers, plasmids, and PCR products used in this study
| Primer pair (5′-3′)a | PCR productb | Plasmid |
|---|---|---|
| GACCGCTTGAAGTCTTTAAT | 403Δ20 | pXYT403Δ20 |
| GGATCCGATTACAATAGCTAAG | ||
| TGGGACGAAGACGAACACTT | 403Δ50 | pXYT403Δ50 |
| GGATCCGATTACAATAGCTAAG | ||
| ACGCCTTGATTGACAAGGAT | 403Δ100 | pXYT403Δ100 |
| GGATCCGATTACAATAGCTAAG | ||
| CTGGATACCGGGAAACGCT | 403Δ200 | pXYT403Δ200 |
| GGATCCGATTACAATAGCTAAG | ||
| CTAAGGGTGTGGCCCTTCCG | LucM0 | |
| TAAAGACTTCAAGCGGTCAA | ||
| TGGGTTACCTAAGGGTGTGGCCTT | LucM8 | |
| TATTTAATAAAGACTTCAAGCGGTC | ||
| ACTGGGTTACCTAAGGGTGTGGCCC | LucM10 | |
| TGTATTTAATTAAAGACTTCAAGCGG | ||
| CTACTGGGTTACCTAAGGGTGTGGC | LucM12 | |
| TTTGTATTTAATTAAAGACTTCAAGC | ||
| ATCTACTGGGTTACCTAAGGGTGTG | LucM14 | |
| CCTTTGTATTTAATTAAAGACTTCAAGC | ||
| GGATCTACTGGGTTACCTAAGGGTG | LucM16 | |
| ATCCTTTGTATTTAATTAAAGACTTCAAGC | ||
| CTGGATCTACTGGGTTACCTAAGGG | LucM18 | |
| ATATCCTTTGTATTTAATTAAAGACTTCAAGC | ||
| CTCTGGATCTACTGGGTTACCTAAG | LucM20 | pXYTLucM20 |
| TGATATCCTTTGTATTTAATTAAAGACTTCAAGC | ||
| TGACAAAACAATTGCACTGATAATG | LucM50 | |
| AATATCGATTCCAATTCAGCGGGG | ||
| CTACCTCCCGGTTTTAATG | LucM100 | |
| CGTCGGGAAGACCTGCCACG | ||
| CGTGCAAAAAAAATTACCAATAA | LucM200 | |
| GTTGTTACTTGACTGGCGACG | ||
| GGCGCGTTATTTATCGGAGTTGCAG | LucM333 | pXYTLucM333 |
| TTTTACAATTTGGACTTTCCGCCCTTC | ||
| GGATCTACTGGGTTACCAGAAACAGGCATCTTACGCGTG | 826-bp SFV | pRP406-S800 |
| ATCCTTTGTATTTAATTAAACGGAAACGCCTTGGT | ||
| GGATCTACTGGGTTACCTCGTTGTCTGAGCTGCCG | 1,621-bp SFV | pRR406-S1600 |
| ATCCTTTGTATTTAATTAAACGGAAACGCCTTGG |
a
Underlined nucleotides overlap with the vector.
b
SFV, Shope fibroma virus.