Fig. 3. Gαi3 silencing by shRNA inhibits pancreatic cancer cell growth, cell cycle progression and mobility.

The primary priPC-1 cells, the immortalized BxPC-3 cells and primary human pancreatic epithelial cells (pEpi1 and pEpi3) underwent stable transfection with lentivirus carrying Gαi3-targeting shRNAs (“sh-Gαi3”, with s1/s2 representing two distinct sequences) or a scramble control shRNA (“shC”), listed genes and proteins were tested (A, B, I, J, N). Cells were further cultivated for applied periods, cell viability (C–K, and P), proliferation (D, L, O), colony formation (E), cell cycle progression (F), migration (G and M), and invasion (H) were assessed. *P < 0.05 versus “shC” group. “N.S.” stands for P > 0.05. The experiments depicted in this figure were replicated five times (n = 5, biological repeats), consistently yielding similar results. Scale bar = 100 μm.