Fig. 6. 25HC increases the cytotoxicity of gilteritinib in lung cancer cells via regulating of cholesterol metabolism.

A A549 and H1650 cells were treated with 100 nM gilteritinib, or 3 μM 25HC, or in combination, cell vitality was determined using crystal violet staining. B A549 and H1650 cells were treated with increasing concentrations of gilteritinib alone or in combination with 3 μM 25HC, and cell vitality was determined by CCK8 assay. C A549 cells were treated with the indicated agents, and intracellular free cholesterol was labeled with filipin staining. D A549 and H1650 cells were treated with the indicated agents, cell vitality was determined using crystal violet staining. E A549 and H1650 cells were treated with indicated agents, and the percentage of cells undergoing apoptosis was tested by flow cytometry. ***P < 0.001.