TABLE 1.
Gag-SN expression in tissues of transgenic mouse lines E and Fa
| Tissue Type |
n
|
Result by:
|
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Northern blotting
|
Immunoblotting
|
Zymogram
|
RT-PCR
|
|||||||
| E (total, 20) | F (total, 24) | E | F | E | F | E | F | E | F | |
| Circulatory | ||||||||||
| Heart | 1 | 1 | − | − | ||||||
| Lung | 1 | 2 | − | − | − | |||||
| Neural | ||||||||||
| Brain (whole) | 2 | 2 | − | − | − | |||||
| Gastrointestinal | ||||||||||
| Intestine | 1 | 1 | − | − | − | − | ||||
| Liver | 2 | 2 | + | + | ||||||
| Genitourinary | ||||||||||
| Kidney | 1 | 2 | + | − | − | |||||
| Testis | 6 | 6 | + | + | + | + | + | |||
| Ovary | 1 | 1 | − | + | ||||||
| Lymphoid | ||||||||||
| LN | 2 | 1 | + | − | ||||||
| Spleen | 1 | 1 | − | − | − | − | + | |||
| Thymus | 1 | 2 | − | + | − | + | + | |||
| B lymphocytes | 1 | 1 | + | − | ||||||
| T lymphocytes | 2 | 1 | + | + | ||||||
| Other | ||||||||||
| Bone marrow | 1 | 1 | + | − | + | |||||
| Skeletal muscle | 1 | 1 | − | − | ||||||
| Skin | 1 | 1 | + | + | ||||||
| Tail tissue | 9 | 11 | + | + | ||||||
a
Twenty line E and 24 line F mice 34 to 548 days old were analyzed for expression of the antiviral gene product by RT-PCR, RNA and immunoblotting analyses, and Zymogram assays. All tissues that tested positive for the antiviral gene product by immunoblot analysis exhibited the 85-kDa Gag-SN fusion protein. Proteolytic cleavage products were not detectable in immunoblot analyses. Zymogram analyses revealed 30- and 18-kDa proteolytic cleavage products in bone marrow, thymus, spleen, and testis. A 58-kDa intermediate product could be detected exclusively in testis (Fig. 2D). −, no expression; +, expression.